Search for: All records

Radiocarbon-dated peat cores collected from an ombrotrophic bog in southern Estonia record shifting environmental conditions and carbon accumulation rates in northern Europe during the late Holocene. Modern observations indicate that the water balance of the peatland is highly influenced by changes in relative humidity, followed by temperature and precipitation. The modern δ18O and δ2H values of surface water suggest that the groundwater is an integration of several months of precipitation. There also appears to be little or no direct influence of surface evaporation on the water within the bog, suggesting that water loss is preferentially through transpiration and sub-surface flow. Bulk peat δ13C values exhibit a trend of higher values through the late Holocene, suggesting a pattern of overall increased surface wetness. The δ15N values were low from ~4130 to 3645 cal yr BP, suggesting drier conditions, followed by intermediate values until ~2995 cal yr BP. The δ15N values decrease again from ~2995 to 2470 cal yr BP, suggesting a return to drier conditions, followed by intermediate values until ~955 cal yr BP. The δ15N values were high, suggesting wetter conditions from ~955 to 250 cal yr BP, followed by intermediate values through the modern. Carbon accumulation rates were low to intermediate from ~4200 to 2470 cal yr BP, followed by intermediate-to-high values until ~1645 cal yr BP. Carbon accumulation rates were then low until ~585 cal yr BP, followed by intermediate values through the modern. The geochemical data, combined with observed changes in peat composition and regional proxies of temperature and water table fluctuations through the late Holocene, suggest that carbon accumulation rates were relatively low under dry and warm conditions, whereas accumulation was generally higher (up to ~80 g C m−2 yr−1) when the climate was wetter and/or colder. These findings further suggest that future environmental changes affecting the regional water balance and temperature will impact the potential for northern peatlands to capture and store carbon. 

ABSTRACT Bacteria have a variety of mechanisms for adapting to environmental perturbations. Changes in oxygen availability result in a switch between aerobic and anaerobic respiration, whereas iron limitation may lead to siderophore secretion. In addition to metabolic adaptations, many organisms respond by altering their cell shape. Caulobacter crescentus , when grown under phosphate-limiting conditions, dramatically elongates its polar stalk appendage. The stalk is hypothesized to facilitate phosphate uptake; however, the mechanistic details of stalk synthesis are not well characterized. We used a chemical mutagenesis approach to isolate and characterize stalk-deficient mutants, one of which had two mutations in the phosphomannose isomerase gene ( manA ) that were necessary and sufficient to inhibit stalk elongation. Transcription of the pho regulon was unaffected in the manA mutant; therefore, ManA plays a unique regulatory role in stalk synthesis. The mutant ManA had reduced enzymatic activity, resulting in a 5-fold increase in the intracellular fructose 6-phosphate/mannose 6-phosphate ratio. This metabolic imbalance impaired the synthesis of cellular envelope components derived from mannose 6-phosphate, namely, lipopolysaccharide O-antigen and exopolysaccharide. Furthermore, the manA mutations prevented C. crescentus cells from efficiently entering stationary phase. Deletion of the stationary-phase response regulator gene spdR inhibited stalk elongation in wild-type cells, while overproduction of the alarmone ppGpp, which triggers growth arrest and stationary-phase entry, increased stalk length in the manA mutant strain. These results demonstrate that sugar-phosphate metabolism regulates stalk elongation independently of phosphate starvation. IMPORTANCE Metabolic control of bacterial cell shape is an important mechanism for adapting to environmental perturbations. Caulobacter crescentus dramatically elongates its polar stalk appendage in response to phosphate starvation. To investigate the mechanism of this morphological adaptation, we isolated stalk-deficient mutants, one of which had mutations in the phosphomannose isomerase gene ( manA ) that blocked stalk elongation, despite normal activation of the phosphate starvation response. The mutant ManA resulted in an imbalance in sugar-phosphate concentrations, which had effects on the synthesis of cellular envelope components and entry into stationary phase. Due to the interconnectivity of metabolic pathways, our findings may suggest more generally that the modulation of bacterial cell shape involves the regulation of growth phase and the synthesis of cellular building blocks. 

ABSTRACT Caulobacter crescentus adapts to phosphate starvation by elongating its cell body and a polar stalk structure. The stalk is an extension of the Gram-negative envelope containing inner and outer membranes as well as a peptidoglycan cell wall. Cellular elongation requires a 6- to 7-fold increase in membrane synthesis, yet phosphate limitation would preclude the incorporation of additional phospholipids. In the place of phospholipids, C. crescentus can synthesize several glycolipid species, including a novel glycosphingolipid (GSL-2). While glycosphingolipids are ubiquitous in eukaryotes, the presence of GSL-2 in C. crescentus is surprising since GSLs had previously been found only in Sphingomonas species, in which they play a role in outer membrane integrity. In this paper, we identify three proteins required for GSL-2 synthesis: CcbF catalyzes the first step in ceramide synthesis, while Sgt1 and Sgt2 sequentially glycosylate ceramides to produce GSL-2. Unlike in Sphingomonas , GSLs are nonessential in C. crescentus ; however, the presence of ceramides does contribute to phage resistance and susceptibility to the cationic antimicrobial peptide polymyxin B. The identification of a novel lipid species specifically produced upon phosphate starvation suggests that bacteria may be able to synthesize a wider variety of lipids in response to stresses than previously observed. Uncovering these lipids and their functional relevance will provide greater insight into microbial physiology and environmental adaptation. IMPORTANCE Bacteria adapt to environmental changes in a variety of ways, including altering their cell shape. Caulobacter crescentus adapts to phosphate starvation by elongating its cell body and a polar stalk structure containing both inner and outer membranes. While we generally think of cellular membranes being composed largely of phospholipids, cellular elongation occurs when environmental phosphate, and therefore phospholipid synthesis, is limited. In order to adapt to these environmental constraints, C. crescentus synthesizes several glycolipid species, including a novel glycosphingolipid. This finding is significant because glycosphingolipids, while ubiquitous in eukaryotes, are extremely rare in bacteria. In this paper, we identify three proteins required for GSL-2 synthesis and demonstrate that they contribute to phage resistance. These findings suggest that bacteria may synthesize a wider variety of lipids in response to stresses than previously observed. 

Arctic sea-ice loss is emblematic of an amplified Arctic water cycle and has critical feedback implications for global climate. Stable isotopes (δ 18 O, δ 2 H, d-excess ) are valuable tracers for constraining water cycle and climate processes through space and time. Yet, the paucity of well-resolved Arctic isotope data preclude an empirically derived understanding of the hydrologic changes occurring today, in the deep (geologic) past, and in the future. To address this knowledge gap, the Pan-Arctic Precipitation Isotope Network (PAPIN) was established in 2018 to coordinate precipitation sampling at 19 stations across key tundra, subarctic, maritime, and continental climate zones. Here, we present a first assessment of rainfall samples collected in summer 2018 ( n = 281) and combine new isotope and meteorological data with sea ice observations, reanalysis data, and model simulations. Data collectively establish a summer Arctic Meteoric Water Line where δ 2 H = 7.6⋅δ 18 O–1.8 ( r 2 = 0.96, p < 0.01). Mean amount-weighted δ 18 O, δ 2 H, and d-excess values were −12.3, −93.5, and 4.9‰, respectively, with the lowest summer mean δ 18 O value observed in northwest Greenland (−19.9‰) and the highest in Iceland (−7.3‰). Southern Alaska recorded the lowest mean d-excess (−8.2%) and northern Russia the highest (9.9‰). We identify a range of δ 18 O-temperature coefficients from 0.31‰/°C (Alaska) to 0.93‰/°C (Russia). The steepest regression slopes (>0.75‰/°C) were observed at continental sites, while statistically significant temperature relations were generally absent at coastal stations. Model outputs indicate that 68% of the summer precipitating air masses were transported into the Arctic from mid-latitudes and were characterized by relatively high δ 18 O values. Yet 32% of precipitation events, characterized by lower δ 18 O and high d-excess values, derived from northerly air masses transported from the Arctic Ocean and/or its marginal seas, highlighting key emergent oceanic moisture sources as sea ice cover declines. Resolving these processes across broader spatial-temporal scales is an ongoing research priority, and will be key to quantifying the past, present, and future feedbacks of an amplified Arctic water cycle on the global climate system.