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Abstract Lipopolysaccharide (LPS) is essential in most Gram-negative bacteria, but mutants of several species have been isolated that can survive in its absence.Caulobacter crescentusviability in the absence of LPS is partially dependent on the anionic sphingolipid ceramide diphosphoglycerate (CPG2). Genetic analyses showed thatccna_01210, which encodes a nucleotidyltransferase, is required for CPG2 production. Using purified recombinant protein, we determined that CCNA_01210 (CpgD) is a phosphoglycerate cytidylyltransferase which uses CTP and 3-phosphoglycerate to produce CDP-glycerate, which we hypothesize is the phosphoglycerate donor for CPG2 synthesis. CpgD had optimum activity at pH 7.5-8 in the presence of magnesium. CpgD exhibited Michaelis-Menten kinetics with respect to 3-phosphoglycerate (Km,app = 10.9 ± 0.7 mM; Vmax,app = 0.72 ± 0.02 µmol/min/mg enzyme) and CTP (Km,app = 4.8 ± 0.9 mM; Vmax,app = 0.44 ± 0.03 µmol/min/mg enzyme). The characterization of this enzyme uncovers another piece of the pathway towards CPG2 synthesis. 

This dataset documents the occurrence, distribution, and characteristics of cryptic ice wedge networks in the Yukon-Kuskokwim Delta (YKD), Alaska. The dataset is derived from remote sensing analyses, field-based permafrost coring, ground-penetrating radar (GPR) surveys, and stable water isotope analyses. High-resolution aerial orthoimagery from 2018 enabled the identification of ~50 linear kilometers (km) of ice wedge trough networks within a 60 square kilometers (km²) study area near Bethel, Alaska, revealing ice wedge networks previously undocumented in the region. Fieldwork in 2023 and 2024 confirmed the presence of ice wedges up to 1.5 meter (m) wide and 2.5 m tall, with wedge tops averaging 0.9 m below the surface. GPR transects identified additional ice wedges beyond those visible in imagery, suggesting that remote sensing analyses may underestimate their true abundance. Coring of polygon centers revealed a suite of late-Quaternary deposits, including early Holocene peat, ice-rich late-Pleistocene permafrost (reworked Yedoma), charcoal layers indicating past tundra fires, and the Aniakchak CFE II tephra (~3,600 calendar years before present [cal yrs BP]). Stable water isotope analyses of wedge ice (mean δ¹⁸O = -15.7 ‰, δ²H = -113.1 ‰) indicate relatively enriched values compared to other Holocene ice wedges in Alaska, reflecting the region's warm maritime climate influence. Expanding the mapping analysis across the YKD using very high-resolution satellite imagery, we found that 95 % of observed ice wedge networks occur at elevations between 4 and 80 meters above sea level (m asl), predominantly within tundra vegetation classes. These areas, covering ~32 % of the YKD tundra region, may contain additional ice wedges, peat deposits, and relict Yedoma. This dataset provides a new framework for understanding the spatial distribution and environmental controls on ice wedge development in warm permafrost regions, with implications for permafrost resilience, climate change vulnerability, and land use planning in the YKD. 

Significance The choroid plexus (ChP) epithelial network displays diverse dynamics, including propagating calcium waves and individuated fluctuations in single cells. These rapid events underscore the possibility that ChP dynamics may reflect behaviorally relevant and clinically important changes in information processing and signaling. Optogenetic and chemogenetic tools provide spatiotemporally precise and sustained approaches for testing such dynamics in vivo. Here, we describe the feasibility of a novel combined opto- and chemogenetic tool, BioLuminescent-OptoGenetics (BL-OG), for the ChP in vivo. In the “LuMinOpsin” (LMO) BL-OG strategy, a luciferase is tethered to an adjacent optogenetic element. This molecule allows chemogenetic activation when the opsin is driven by light produced through luciferase binding a small molecule (luciferin) or by conventional optogenetic light sources and BL-OG report of activation through light production. Aim To test the viability of BL-OG/LMO for ChP control. Approach Using transgenic and Cre-directed targeting to the ChP, we expressed LMO3 (a Gaussia luciferase-VChR1 fusion), a highly effective construct in neural systems. In mice expressing LMO3 in ChP, we directly imaged BL light production following multiple routes of coelenterazine (CTZ: luciferin) administration using an implanted cannula system. We also used home-cage videography with Deep LabCut analysis to test for any impact of repeated CTZ administration on basic health and behavioral indices. Results Multiple routes of CTZ administration drove BL photon production, including intracerebroventricular, intravenous, and intraperitoneal injection. Intravenous administration resulted in fast “flash” kinetics that diminished in seconds to minutes, and intraperitoneal administration resulted in slow rising activity that sustained hours. Mice showed no consistent impact of 1 week of intraperitoneal CTZ administration on weight, drinking, motor behavior, or sleep/wake cycles. Conclusions BL-OG/LMO provides unique advantages for testing the role of ChP dynamics in biological processes. 

ABSTRACT The Yukon‐Kuskokwim Delta (YKD), covering ~75,000 km²of Alaska's discontinuous permafrost zone, has a historic (1902–2023) mean annual air temperature of ~−1°C and was previously thought to lack ice wedge networks. However, our recent investigations near Bethel, Alaska, revealed numerous near‐surface ice wedges. Using 20 cm resolution aerial orthoimagery from 2018, we identified ~50 linear km of ice wedge troughs in a 60 km²study area. Fieldwork in 2023 and 2024 confirmed ice wedges up to ~1.5 m wide and ~2.5 m in vertical extent, situated on average 0.9 m below the tundra surface (n = 29). Ground‐penetrating radar (GPR) detected additional ice wedges beyond those visible in the remote sensing imagery, suggesting an underestimation of their true abundance. Coring of polygonal centers revealed late‐Quaternary deposits, including thick early Holocene peat, late‐Pleistocene ice‐rich silts (reworked Yedoma), charcoal layers from tundra fires, and the Aniakchak CFE II tephra (~3600 cal yrs BP). Stable water isotopes from Bethel's wedge ice (mean δ¹⁸O = −15.7 ‰, δ²H = −113.1 ‰) indicate a relatively enriched signature compared to other Holocene ice wedges in Alaska, likely due to warmer temperatures and maritime influences. Expanding our mapping across the YKD using high‐resolution satellite imagery from 2012 to 2024, we estimate that the Holocene ice wedge zone encompasses ~30% of the YKD tundra region. Our findings demonstrate that ice wedge networks are more widespread across the YKD than previously recognized, emphasizing both the resilience and vulnerability of the region's warm, ice‐rich permafrost. These insights are crucial for understanding permafrost responses to climate change and assessing agricultural potential and development in the region.